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KMID : 0613820120220060723
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Journal of Life Science
2012 Volume.22 No. 6 p.723 ~ p.729
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Filter Plate Micro Trap as a Device for in situ Cultivation for Environmental Microorganisms(
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Jung Da-Woon
Ahn Tae-Seok
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Abstract
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Filter plate microbial trap (FPMT) was invented as an in situ cultivation device for the isolation of bacteria from natural environments. FPMT consists of a medium and membrane filters (0.45 ㎛ pore size) and microorganisms and compounds can be moved freely moved into the medium. This device was applied to two soil samples of Greenland. The microbial diversity of both soil samples by FPMT was higher than that by the conventional Petri dish-based method. Moreover, novel bacterial species were isolated by FPMT. The new FPMT is effective for in situ cultivation of natural samples and could be applicable to the isolation of uncultivable microorganism.
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KEYWORD
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Cultivation device, in situ cultivation, microbial diversity, uncultivable bacteria
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